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KMID : 0613820060160030433
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Journal of Life Science
2006 Volume.16 No. 3 p.433 ~ p.441
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Polyamine Induces Apoptosis Through the Calcium Signaling in Human Prostate Cancer Cells
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Song Hwi-June
Kim Ji-Young
Yoo Mi-Ae
Chung Hae-Young
Kim Jong-Min
Kim Byeong-Gee
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Abstract
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Polyamines are essential for the normal cell growth and differentiation. They are also known to have paradoxical dual effects on cell proliferation. In this paper we show that the excess amount of polyamines induces apoptosis through the modulation of calcium signaling in LNCaP human prostate cancer cells. Polyamines, particularly spermidine and spermine, stimulated cell proliferation at a lower concentration (under 10 μM), but it inhibited cell viability at a higher concentration (40 μM). The levels of intracellular Ca²? concentration were increased only at a high concentration of polyamines treatment without any noticeable changes at lower concentrations. Nifedipine did not alter the increase of polyamine-induced Ca²? levels, but flufenamic acid totally abolished the increase of intracellular Ca²? levels. These results mean that polyamines induce Ca²? influx from the surroundings through nonselective cation channels on the cell membrane. The expression of Bcl-2 protein was almost completely blocked, but the level of Bax protein was increased dramatically in the cells treated with high concentration of polyamine. The present study shows that polyamines at a high concentration induce apoptosis through the modulation of intracellular calcium signaling. The increase of intracellular calcium level induced by polyamines, was possibly a result from the extracellular calcium influx through the nonselective cation channels.
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KEYWORD
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Apoptosis, Bcl-2, Bax, calcium, polyamines
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